Effect of Holothuria atra Body Wall Extract on Pulmonary Fibrosis and Oxidative Stress caused by Bleomycin in Rat

Authors
Hassan Mehdizadeh Kakayae
Ali Hosseini Sharifabad
Afsaneh Yegdaneh
Adel Mohammad Alipour
10.61186/ibj.25th-11th-IACRTIMSS
Abstract
Introduction: Idiopathic pulmonary fibrosis leads to the misplaced deposition of the extracellular matrix and stiffness parenchyma and the creation of scarring (fibrosis) in the lung. This study was conducted to investigate the effectiveness of different doses of Holothuria atra body wall extract on bleomycin (BLM)-induced pulmonary fibrosis.

Methods and Materials: Animals were randomly divided into six groups (n = 6). The control group received normal saline intratracheally as a single dose on day one. The BLM group received a single intratracheal dose of BLM (7.5 IU/kg) along with daily doses of normal saline by gavage. The pirfenidone group received a single intratracheal dose of BLM (7.5 IU/kg) and daily doses of pirfenidone by gavage. The remaining groups also received a single intratracheal dose of BLM (7.5 IU/kg) and were administered daily doses of H. atra extract at 50, 100, and 200 mg/kg. Finally, the rats were euthanized, and the lung tissues were taken out for histological analysis and biochemical assessments.

Results: Histological tests showed that BLM could induce marked pulmonary fibrosis within two weeks. Administration of a hydroalcoholic extract of H. atra body wall reduced these damages in lung tissue in a dose-dependent manner. The best results were obtained with the consumption of 200 mg/kg per day of the extract of H. atra body wall.

Conclusion and Discussion: The findings of the present study suggest that the extract of H. atra body wall can reduce the toxic effects of BLM on lung tissues. Such an effect of H. atra can be attributed to the compounds of this plant, which have anti-inflammatory and antioxidant properties.


Keywords
Bleomycin sulfate
Holothuria atra
Oxidative stress
Pulmonary fibrosis