CRISPR-Cas Technology as a Revolutionary Genome Editing tool: Mechanisms and Biomedical Applications

Authors
Sahar Ebrahimi 1
Mohammad Ali Khosravi 2
Abbasali Raz 3
Morteza Karimipoor 2
Parviz Parvizi* 1
1 Molecular Systematics Laboratory, Parasitology Department, Pasteur Institute of Iran, Tehran, Iran
2 Molecular Medicine Department, Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran
3 Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran
10.61186/ibj.3898
Abstract
Programmable nucleases are powerful genomic tools for precise genome editing. These tools precisely recognize, remove, or change DNA at a defined site, thereby stimulating cellular DNA repair pathways that can cause mutations or accurate replacement or deletion/insertion of a sequence. The CRISPR-Cas9 system is the most potent and useful genome editing technique adapted from the immune system of certain bacteria and archaea against viruses and phages. In the past decade, this technology has made notable progress, and at present, it has largely been used in genome manipulation to make precise gene editing in plants, animals, and human cells. In this review, we aimed to explain the basic principles, mechanisms of action, and applications of this system in different areas of medicine, with an emphasize on the detection and treatment of parasitic diseases.
Keywords
CRISPR-Cas Systems
Gene editing
Medicine
parasitology