Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber

Authors
Atena Shapourzadeh 1
Seyed Mohammad Atyabi 2
Shiva Irani 3
Hadi Bakhshi 4
1 Department of Biochemistry, Faculty of Basic Science, Islamic Azad University Damghan Branch, Damghan, Iran
2 Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran 13164, Iran
3 Science and Research Branch, Islamic Azad University, Tehran, Iran
4 Universitatsstraße Bayreuth, Germany-Universitatsstraße Bayreuth, Germany
10.29252/ibj.24.4.236
Abstract
Background: Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes. Methods: In this work, carboxymethyl chitosan (CMC; 20%) was grafted to Polycaprolactone (PCL) nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface. Results: The results of Fourier Transform Infrared Spectroscopy confirmed CMC (20%) graft on PCL scaffold. The Thiazolyl blue tetrazolium bromide assay showed a significant enhancement (p < 0.05) in the cell affinity and proliferation of adipose-derived stem cells (ADSCs) to CMC20%-graft-PCL scaffolds. After 14 days, bone differentiation was affirmed through alizarin red and calcium depositions. Conclusion: Based on the results, the CMC20%-graft-PCL can support the proliferation of ADSCs and induce the differentiation into bone with longer culture time.
Keywords
carboxymethyl chitosan
Mesenchymal stem cells
Tissue engineering