Background: DNA polymerase β (pol β) is a key enzyme of base excision repair pathway. It is a 1-kb gene consisting of 14 exons. Its catalytic part lies between exon 8 and exon 14. Exon 12 has a role in deoxyribonucleotide triphosphate selection for nucleotide transferase activity. Methods: Genomic DNA was isolated from ovarian carcinoma samples. Single strand conformation polymorphism method was used to detect mutation in genomic DNA. Results: Twenty-four patients of the 152 pair of tumor samples (15.8%) exhibited a point mutation (C→G) in position 725 in exon 12, which shifts proline to arginine (P242R). Statistical analysis showed a significant (p < 0.001) relationship between pol β mutation and the age of detection. Conclusion: This is a newly reported somatic mutation of pol β in ovarian carcinoma patients from India.
Khanra,K , Choudhuri,I and Bhattacharyya,N . (2018). Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples. Iranian Biomedical Journal, 22(5), 355-359. doi: 10.29252/ibj.22.5.355
MLA
Khanra,K , , Choudhuri,I , and Bhattacharyya,N . "Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples", Iranian Biomedical Journal, 22, 5, 2018, 355-359. doi: 10.29252/ibj.22.5.355
HARVARD
Khanra K, Choudhuri I, Bhattacharyya N. (2018). 'Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples', Iranian Biomedical Journal, 22(5), pp. 355-359. doi: 10.29252/ibj.22.5.355
CHICAGO
K Khanra, I Choudhuri and N Bhattacharyya, "Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples," Iranian Biomedical Journal, 22 5 (2018): 355-359, doi: 10.29252/ibj.22.5.355
VANCOUVER
Khanra K, Choudhuri I, Bhattacharyya N. Detection of Somatic Mutation in Exon 12 of DNA Polymerase β in Ovarian Cancer Tissue Samples. Iranian Biomedical Journal. 2018;22(5):355-359. doi: 10.29252/ibj.22.5.355
Iranian Biomedical Journal