Molecular Screening and Single Nucleotide Polymorphism Typing of Molluscum Contagiosum Virus (MCV) from Genital Specimens, between 2012 and 2015

Authors
Sedigheh Taghinezhad-S
Amir Hossein Mohseni
Hossein Keyvani
Narges Ghobadi
Research and Development (R&D) Department, Keyvan Virology Specialty Laboratory (KVSL), Tehran, Iran
10.22034/ibj.22.2.129
Abstract
Background: The present study is the first comprehensive report of the Molluscum contagiosum virus (MCV) in Iran based on the molecular technique for differentiation and typing of the MCV1 and MCV2. Methods: Patients were diagnosed as having tumor-like genital warts less than 5 mm in diameter, and HIV seronegative samples were chosen for this cross-sectional study. TaqMan real-time PCR was used to identify MCV following clinical examination. Typing of the MCV-positive specimens was performed in the SNP A27451G region of MC021L gene. Results: Of 1470 samples, 114 (7.75%) samples were positive for the MCV. From MCV-positive samples, 71.05% sequences were found to be related to the MCV1 and 28.95% to the MCV2. Conclusion: This assay constitutes a reliable method for identification and typing of the MCV genomic variants that could be valuable for

reviewing the pathogenesis, molecular epidemiology, and the natural history of MCV-related situations.
Keywords
Molluscum contagiosum virus
Polymerase chain reaction
single nucleotide polymorphism