Low Level of Autophagy-Related Gene 10 (ATG10) Expression in the 6-Hydroxydopamine Rat Model of Parkinson's Disease

Authors
Marzieh Shams Nooraei 1
Ali Noori-Zadeh 2
Shahram Darabi 1
Farzad Rajaei 1
Zohreh Golmohammadi 1
Hojjat Allah Abbaszadeh 3
1 Cellular and Molecular Research Center, Qazvin University of Medical Science, Qazvin, Iran
2 Department of Clinical Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences, Ilam,Iran
3 Hearing Disorder Research center, Shahid Beheshti University of Medical Science,Tehran, Iran
10.22034/ibj.22.1.15
Abstract
Background: Autophagy is a mechanism disassembling the damaged organelles from the cell. This study attempted to examine the expression of several autophagy-related genes in Parkinson’s disease (PD) rat model. Methods: The male Wistar rats were divided into three groups as control, sham, and lesion. In the latter group, the PD rat model was induced by the injection of 6-hydroxydopamine in the striatum. The behavioral test was conducted one (baseline) and four weeks after the surgery through apomorphine hydrochloride. Then the RT-PCR technique was employed to evaluate the expressions of p62/SQSTM, autophagy-related genes (ATG)5, ATG12, ATG16L1, ATG10, as well as GAPDH and LC3. Results: By injecting apomorphine, the striatal lesion group showed a significant contralateral rotation at fourth week as compared to the baseline. The examination of p62, ATG5, ATG12, ATG16L1, and LC3 expressions using RT-PCR revealed that p62, ATG5, ATG12, LC3, and ATG16L1 were expressed in the substantia nigra of PD rat model, while ATG10 was not expressed. Conclusion: ATG10 expression is necessary for the initiation of autophagy. Thus, these results show that autophagy deregulation occurs in the initiation stages of the process in the rat model of PD.
Keywords
PARKINSON’S DISEASE
autophagy
6-hydroxydopamine (6-OHDA)
ATG16L
ATG10