E6-Specific Detection and Typing of Human Papillomaviruses in Oral Cavity Specimens from Iranian Patients

Authors
Hadi Razavi Nikoo 1
Abdollah Ardebili 1
Mehrdad Ravanshad 2
Fatemeh Rezaei 2
Ali Teimoori 3
Sayyad Khanizadeh 4
Mohammad Hassan Pouriayevali 5
Mehdi Ajorloo 4
1 Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran
2 Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3 Health Research Institute, Infectious and Tropical Diseases Reseach Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4 Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
5 Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
10.18869/acadpub.ibj.21.6.411
Abstract
Background: Detection and quantification of human Papillomavirus (HPV) genome in oral carcinoma play an important role in diagnosis, as well as implications for progression of disease. Methods: We evaluated tissues from 50 esopharyngeal cancers collected from different regions of Iran for HPV E6 using the two type-specific primers sets. E6 gene of HPV genotypes was amplified by specific primers. The sensitivity of PCR assay was analyzed and determined using HPV-DNA-containing plasmids. Real-time PCR was utilized to determine the prevalence and HPV viral load in patients with oral cavity squamous cell carcinoma. Results: Eighteen (36%) specimens were positive for HPV. Among the 18 positive specimens, 10 showed HPV-18 (55.55%), and 8 specimens were positive for HPV-11 (44.44%). Of the 18 infected specimens, 6 (33.32%) and 12 (66.65%) were identified as high-titer and low-titer viral load, respectively. Conclusions: The PCR-based assay, developed in the current study, could be used for HPV detection, quantification, and genotyping in epidemiological and clinical studies.
Keywords
Real-time PCR
genotyping
Iran