DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with polyethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and fractionated by gel filtration. The resulting enzyme preparation was stable and active.
Bouzari,S and Rechinsky,V O . (1998). Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector. Iranian Biomedical Journal, 2(2), 79-82.
MLA
Bouzari,S , and Rechinsky,V O . "Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector", Iranian Biomedical Journal, 2, 2, 1998, 79-82.
HARVARD
Bouzari S, Rechinsky V O. (1998). 'Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector', Iranian Biomedical Journal, 2(2), pp. 79-82.
CHICAGO
S Bouzari and V O Rechinsky, "Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector," Iranian Biomedical Journal, 2 2 (1998): 79-82,
VANCOUVER
Bouzari S, Rechinsky V O. Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector. Iranian Biomedical Journal. 1998;2(2):79-82.
Iranian Biomedical Journal