Prostate-specific antigen (PSA) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a CM-Sephadex C-50 and by gel filtration on a Sephacryl S-200 column. A single 33-kDa protein band appeared in SDS-PAGE. High pressure liquid chromatography (HPLC) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five different isoforms of this protein. The immunoreactivity of the purified PSA was checked by Western blotting. This simple two-step method can be used for a large-scale preparation of the purified PSA for the clinical tests and also for further investigative studies on the biological properties of this protein.
Kamali Sarvestani,E , Khezri,A , Vessal,M and Ghaderi,A . (1999). A Simplified and Reproducible Two-Step Method for the Purification of Prostate-Specific Antigen. Iranian Biomedical Journal, 3(3), 89-91.
MLA
Kamali Sarvestani,E , , Khezri,A , , Vessal,M , and Ghaderi,A . "A Simplified and Reproducible Two-Step Method for the Purification of Prostate-Specific Antigen", Iranian Biomedical Journal, 3, 3, 1999, 89-91.
HARVARD
Kamali Sarvestani E, Khezri A, Vessal M, Ghaderi A. (1999). 'A Simplified and Reproducible Two-Step Method for the Purification of Prostate-Specific Antigen', Iranian Biomedical Journal, 3(3), pp. 89-91.
CHICAGO
E Kamali Sarvestani, A Khezri, M Vessal and A Ghaderi, "A Simplified and Reproducible Two-Step Method for the Purification of Prostate-Specific Antigen," Iranian Biomedical Journal, 3 3 (1999): 89-91,
VANCOUVER
Kamali Sarvestani E, Khezri A, Vessal M, Ghaderi A. A Simplified and Reproducible Two-Step Method for the Purification of Prostate-Specific Antigen. Iranian Biomedical Journal. 1999;3(3):89-91.
Iranian Biomedical Journal