A sensitive high performance liquid chromathography (HPLC) analytical procedure was developed for the quantitive determination of trimethoprim (TM* and sulphamethoxazole (SM) in commercial dosage forms. C18 analytical column (stainless steel, 25 cm * 4.6 mm i.d.) was packed with 5-*m particles of the reversed phase material and used for assays. Mobile phase containing 0.025 M sodium phosphate as aqueous phase and acetonitrile with 0.4% triethylamine as organic phase. The drugs were quantified at flow-rate of 1.2 ml/min, with ultraviolet detection at 260 nm. The minimum detectable quantities in assays were 100 ng/ml for SM and 75 ng/ml for TM. The method is well suited to routine application and adequate sensitivity with precision.
Rezaee,A , Behzadian Nejad,Q and Kebriaeezadeh,A . (2000). Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography. Iranian Biomedical Journal, 4(2), 75-78.
MLA
Rezaee,A , , Behzadian Nejad,Q , and Kebriaeezadeh,A . "Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography", Iranian Biomedical Journal, 4, 2, 2000, 75-78.
HARVARD
Rezaee A, Behzadian Nejad Q, Kebriaeezadeh A. (2000). 'Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography', Iranian Biomedical Journal, 4(2), pp. 75-78.
CHICAGO
A Rezaee, Q Behzadian Nejad and A Kebriaeezadeh, "Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography," Iranian Biomedical Journal, 4 2 (2000): 75-78,
VANCOUVER
Rezaee A, Behzadian Nejad Q, Kebriaeezadeh A. Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography. Iranian Biomedical Journal. 2000;4(2):75-78.
Iranian Biomedical Journal