Time Course of Degradation and Deadenylation of Maternal c-mos and Cyclin A2 mRNA during Early Development of One-Cell Embryo in Mouse

Alizadeh, Zohreh; Pasbakhsh, Parichehr; Sobhani, Aligholi; Barbarestani, Mohammad; Ghafari, Marefat; Etesam, Faride

Time Course of Degradation and Deadenylation of Maternal c-mos and Cyclin A2 mRNA during Early Development of One-Cell Embryo in Mouse

Authors

Zohreh Alizadeh

Parichehr Pasbakhsh

Aligholi Sobhani

Mohammad Barbarestani

Marefat Ghafari

Faride Etesam

Abstract

Early in the development of many animals, before transcription begins, any change in the pattern of protein synthesis is attributed to a change in the translational activity or stability of mRNA in the egg and early embryo. As a result, translational control is critical for a variety of developmental decisions, including oocyte maturation and initiation of preimplantation development. In this study, using real-time RT-PCR method, we defined the time course of degradation and deadenylation of an oocyte specific gene (c-mos) more precisely and a gene that is re-synthesized after ZGA (cyclin A2). Our data indicate that oocyte-specific transcript, c-mos, degrades rapidly while cyclin A2 mRNA does not and the deadenylation of c-mos mRNA precedes the process of degradation. Our findings suggest that time-dependent elimination of different maternal mRNA is a way for regulation of translation in early development of mouse embryos

Keywords

Maternal mRNA

MOUSE EMBRYO

mRNA degradation

c-mos

Cyclin A2