Discrimination of Dominant H. pylori Strains Isolated from Patients with Different Gastroduodenal Pathologies by Protein Profiling

Authors
Shohreh Farshad
Abdolvahab Alborzi
Aziz Japoni
Masoumeh Hayati
Mehdi Saberfirouzi
Kamran Bagheri Lankarani
AliReza Taghavi
Jalil Nasiri
Noreddin Rafatpour
Abstract
It is not clear what factors determine divergent outcomes of infections caused by H. pylori . In the present study, the protein profiles of different strains of H. pylori, isolated from three groups of patients with ulcerative disease, non-ulcerative gastritis and cancer disease, were analyzed using 1DSDS-PAGE. The patterns of different H. pylori strains were highly divergent. About 30.76% (7 bands) of the 26 observed protein bands were common in all strains isolated from 3 groups of the patients. While the similarity for the strains inside each group were 75% (15 from 20), 76.47% (13 from 17) and 78.57% (11 from 14) for cancerous, ulcerative and nonulcerative group, respectively. Some of the observed bands were significantly specific for each group. Therefore, we speculated that some H. pylori strains might be more associated with a specific disease than others, giving the clustering of some, but not all, strains within each disease group. In conclusion, this study showed that protein profile can be a characteristic in discrimination of dominant strains in different gastric clinical status. Specific and dominant proteins of different strains isolated from three groups of patients under study were candidates for further exploration for laboratory tests, which analyze disease-specific H. pylori strains, and for diagnosis of the different diseases and outcomes associated with this widespread bacterium.
Keywords
Helicobacter pylori
SDS-PAGE
Protein profile